• The test involves administering agents (such as insulin, arginine, clonidine, glucagon, or L-dopa) that stimulate GH release.
• Blood samples are collected at multiple time intervals to measure GH levels.
• Low GH levels in response to stimulation may indicate GH deficiency.

Risk Assessment

• Hypoglycemia: Common if insulin is used, which can cause symptoms like dizziness, sweating, or confusion.
• Nausea/Vomiting: Potentially caused by other agents like arginine or glucagon.
• Hypotension: Can occur with clonidine.
• Rare Reactions: Severe hypoglycemia, anaphylaxis to agents used.

Due to these risks, the test is typically conducted in a clinical setting with emergency support available.

GH response varies by age and gender.

Children: Typically, a peak GH level >10 ng/mL is considered normal.

Adults: A peak GH level >5 ng/mL is usually normal, though this can vary by laboratory standards.

Normal Response: Adequate GH levels after stimulation suggest a functional pituitary.

Low Response: A GH level below the normal threshold may indicate GH deficiency.

False Positives/Negatives: Conditions like obesity or stress may affect GH levels, so the test may be repeated if results are inconclusive.

Blood samples are collected in serum tubes at baseline and at intervals (e.g., every 15–30 minutes) after administration of the stimulating agent.