This test detects the genetic material (RNA) of Dengue and Chikungunya viruses using Polymerase Chain Reaction (PCR) technology. It is highly sensitive and specific, especially useful in the early stage of infection (first 1–5 days of fever).

Risk Assessment / Clinical Use:

• Early diagnosis of acute Dengue or Chikungunya infection

• Helps differentiate between Dengue and Chikungunya (which have overlapping symptoms)

• Useful in outbreak surveillance and travel-related fever cases.

Normal Range:

Negative for both Dengue and Chikungunya viral RNA

Interpretation:

• Positive for Dengue RNA → Active dengue infection

• Positive for Chikungunya RNA → Active chikungunya infection

• Negative result → No detectable viral RNA; could indicate no infection, or testing done too late (after viremia phase)

Sample Type:

Blood (Serum or Plasma) – collected in an EDTA or serum tube